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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Anal Biochem. 2006 Dec 5;361(1):47–54. doi: 10.1016/j.ab.2006.11.021

Table 1.

Summary of tumor imaging results

IRDye®800CW EGF Normalized fluorescence Signal loss
Preparation Source Intact animalsa Tumor sectionsb Intact animals Tumor sections
FPLC A 1039.30 94.23
FPLC B 512.77 113.39
HPLC A 746.22 69.58 28%c 26%
HPLC B 185.94 55.92 (64%)d (51%)
HPLC C 100.21 59.44 90% (80%) 37% (48%)
HPLC D 85.63 55.75 92% (83%) 41% (51%)
a

Normalized fluorescence for intact animals: mean pixel fluorescence (tumor) minus mean pixel fluorescence (background).

b

800nm fluorescence per area (mm2).

c

Comparison to Source A FPLC to determine % Loss.

d

Comparison to Source B FPLC to determine % Loss (numbers in parentheses).