Table 1.
IRDye®800CW EGF | Normalized fluorescence | Signal loss | |||
---|---|---|---|---|---|
Preparation | Source | Intact animalsa | Tumor sectionsb | Intact animals | Tumor sections |
FPLC | A | 1039.30 | 94.23 | ||
FPLC | B | 512.77 | 113.39 | ||
HPLC | A | 746.22 | 69.58 | 28%c | 26% |
HPLC | B | 185.94 | 55.92 | (64%)d | (51%) |
HPLC | C | 100.21 | 59.44 | 90% (80%) | 37% (48%) |
HPLC | D | 85.63 | 55.75 | 92% (83%) | 41% (51%) |
Normalized fluorescence for intact animals: mean pixel fluorescence (tumor) minus mean pixel fluorescence (background).
800nm fluorescence per area (mm2).
Comparison to Source A FPLC to determine % Loss.
Comparison to Source B FPLC to determine % Loss (numbers in parentheses).