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pKa Values of the Thiol Group of Enzyme-Bound Glutathione in rGSTA1-1 and Site-Directed Mutants
| enzyme | pKa of GSH | enzyme | pKa of GSH |
|---|---|---|---|
| W21F | 7.46 ± 0.06 | W21F:F220Y | 7.02 ± 0.05 |
| F10Y:W21F | 7.38 ± 0.08 | W21F:F220I | 8.32 ± 0.04 |
| F10A:W21F | 7.76 ± 0.18 | Δ209–222 | 7.72 ± 0.11 |
| F10L:W21F | 7.73 ± 0.13 |
