Table 2.
WM sample concentration (mg/ml) | Rg (Å) from Guinier | Rg (Å) from P(r) | Dmax (Å) | I(0) cm−1 from P(r) | MW (Da) |
---|---|---|---|---|---|
2.0 | 37±1 | 38±1 | 125 | 0.10±0.01 | 67,000±5000 |
1.0 | 34±1 | 35±1 | 110 | 0.041±0.002 | 54,000±5000 |
0.5 | 33±2 | 35±1 | 110 | 0.019±0.001 | 51,000±4000 |
Errors on Rg and I(0) values are standard errors of the mean, taken from several fits to the data in the case of both Guinier and P(r) analyses. Mw values are obtained from equations (1) and (2) using the I(0) values from the P(r) analysis. Errors on the concentration values are assumed to be 5%. Dmax represents the optimal value obtained by testing several values of Dmax in the P(r) analysis and choosing the value that best fit the data and gave an Rg value in agreement with that from Guinier analysis.