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. Author manuscript; available in PMC: 2007 May 10.
Published in final edited form as: J Biol Chem. 2006 Oct 23;281(51):39444–39454. doi: 10.1074/jbc.M608056200

FIGURE 5.

FIGURE 5

Phosphate release kinetics. A, a preformed MT·Eg5-513 complex plus MDCC-PBP was mixed in the stopped-flow instrument with MgATP. Final concentrations: 0.5 μm Eg5-513, 2 μm MTs, 5 μm MDCC-PBP, and 10 μm MgATP. The transient displays an amplitude corresponding to 3 μm Pi with kobs = 0.22 ± 0.0002 s−1. B, a preformed MT·Eg5-513 complex plus MDCCPBP was mixed in the stopped-flow instrument withMgATPat single turnover conditions. Final concentrations: 15 μm Eg5-513, 20 μm MTs, 20 μm MDCCPBP, and 1 μm MgATP. The data were fit to a single exponential function with kobs = 1.9 ± .002 s−1 with an amplitude of 0.98 μm. A and B, insets, the KH2PO4 standard curves used to convert fluorescence voltage to micromolar Pi released for the corresponding experiments.