Figure 3.

TIMP expression in BDL livers. Northern blot analysis of total RNA from rat liver tissue at 2, 5, and 10 days after BDL (A) and 10, 20, and 30 days after BDL (B). Ten micrograms RNA from normal liver (C) was included as a control in addition to mouse (10T1/2) and rat (Rat 1A) fibroblasts either unstimulated (U) or phorbol ester (phorbol myristate acetate) treated. Filters were hybridized to nick-translated mouse TIMP-1, -2, and -3 probes as described in Materials and Methods. As a loading control, the filters were stained with methylene blue, which allows visualization of 28 S and 18 S rRNAs.