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. 1998 Dec;153(6):1947–1956. doi: 10.1016/S0002-9440(10)65708-2

Figure 4.

Figure 4.

Jurkat cells grown in nonconditioned media (A) and in media conditioned with TC-248 cells in the absence (B) or presence (C) of the FasL-neutralizing NOK-2 antibody were stained with the TUNEL method to detect apoptosis. Fluorescent nuclei corresponding to apoptotic nuclei are present in Jurkat cells incubated with TC-248 conditioned media, verifying the existence of functional s-FasL. Jurkat cells in non conditioned media (A) and in conditioned media with blocking antibody (C) show no apoptotic nuclei in these figures. Scant TUNEL-positive cells were only present in these conditions (Magnification, ×130).