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Expression of VEGF/VPF mRNA is not elevated in FGF-transfected cells. RNAse protection of 30 μg of RNA from the indicated cell lines shows that the parental ML-20 cells and the two FGF transfectants have similar levels of mRNA for all isoforms of VEGF/VPF detected. Isoforms detected by the riboprobe are as indicated. The VEGF-165 isoform and the GAPDH band were quantitated by phosphorimaging and VEGF-165/GAPDH ratios calculated and compared.
