Table 1.
In Vivo Growth Characteristics of the Clonal Cell Lines Used in This Study
| Cell line (transfected factor) | Ovariectomized | Estrogen | Tamoxifen |
|---|---|---|---|
| ML-20 (a LacZ-transfected clonal line of MCF-7) | − | + | ± |
| MPCX (control transfection for MV165-14) | − | +++ | ± |
| MKL-4 (FGF-4) | +++ | ++ | +++++ |
| MKL-F (FGF-4) | +++ | ++++ | +++ |
| Clone 18 (FGF-1) | +++ | ++++ | +++ |
| MV165-14 (VEGF/VPF) | − | +++ | ++ |
The table summarizes results of experiments done previously with the indicated cell lines in which tumors were harvested after several months of tumor growth. 10,11,13,15,16 ML-20 and MPCX, lacZ-transfected MCF-7 cell lines, have growth characteristics typical of wild-type MCF-7 cells; ie, they are estrogen-dependent for tumor growth. The transfected angiogenic factors are indicated in parentheses following the names of the clonal cell lines. Symbols are interpreted as follows: −, no tumors are produced; ±, static tumor nodules are produced; + to +++++, actively growing tumors are produced with relative sizes indicated by the number of pluses. Approximate tumor sizes are as follows: +, <30 mm3; ++, 30 to 150 mm3; +++, 150 to 350 mm3; ++++, 350 to 750 mm3; +++++, >750 mm3 after 2 months of tumor growth.