Table 2.
Residues in the helix-helix interfaces of aquaporin crystal structures. Residue pairs that occur at the helix-helix interface in the six high-resolution aquaporin crystal structures.
| Helix Pairs | Residues from the crystal structuresa | <dist.>b(Å) |
| TM1...TM2 | S28...F58, G25...W48, G19...F43, T55...F81,G27...F48, G22...F62 | 4.86 |
| TM1...TM3 | M21...Q103, T18...Q93, T12...Q88, T48...Q126, T20...Q93, T15...Q107 | 5.74 |
| F18...G106, F15...G96, C9...G91, F45...G129, F17...G96, F12...G110 | 4.55 | |
| I25...A107, I22...A97, V16...G92 L52...A130, V24...A97, V19...A111 | 5.70 | |
| I25...A110, I22...A100, V16...A95, L52...G133, V24...G100, V19...G114 | 5.52 | |
| TM4...TM6 | T148...P218, T156...P240, S142...P212, T172...P249, T138...P208, T154...P229 | 5.29 |
| A157...Y229, A165...Y251, G151...Y223, S181...H260, A147...Y219, G163...Y240 | 7.73 | |
| L141/I145...G217, F149/M153...G239, L135/L139...V211, L165/I169...G248, T131/I135...G207, M147/V151...G228 | 5.31 | |
| I145/L149...G221, M153/A157...G243, L139/A143...G215, I169/F173...G252, I135/L139...G211, V151/F155...G232 | 4.35 | |
| L149/V152...A225, A157/M160...G247, A143/L146...G219, F173/V176...A256, L139/V142...G215, F155/M158...A236 | 5.17 | |
| V152...A222, M160...A244, L146...G216, V176...A253, V142...A212, M158...A233 | 5.51 | |
| TM2...TM5 | S55...L183, S45...A192, A40...L175, A78...L211, A45...L173, G59...T188 | 5.91 |
| G59...G175,G59...G184,G44...G167, G82...G203,G49...G165,G63...G180 | 4.31 | |
| LB...TM4 | G84...V152, A74...M160, G69...L146, G107...V176, A74...V142, G88...M158 | 6.96 |
| LB...TM6 | A80...A222,A70...A244,A65...G216, A103...A253,A70...A212,A84...A233 | 5.75 |
| LE...TM3 | G200...A107,G209...A97,A192...G92, G228...A130,A190...A97,G205...A111 | 4.89 |
| LE...TM6 | S198...F214, D207...P236, S190...F208, S226...F245,S188...Y204, T203...Y225 | 6.52 |
a Residue pairs are given respectively for the crystal structures 1J4N, 1FX8, 1RC2, 1Z98, 2B6O and 2F2B. For each residue given for 1J4N, the equivalent residues from the other crystal structures were obtained from structure-based sequence alignment (see Additional files 1 to 8). For example, the equivalent residue pairs for (S28, F58) in 1J4N are (G25, W48) in 1FX8, (G19, F43) in 1RC2, (T55, F81) in 1Z98, (G27, F48) in 2B6O and (G22, F62) in 2F2B. Small residues (Gly/Ala/Ser/Thr) are shown in bold. b Average Cα...Cα distances calculated for residue pairs from all the six crystal structures are given.