Table 3.
Conservation of small and weakly polar residues at the helix-helix interfaces of plant MIP proteins
| Residuea | All families b, c | PIPsc | TIPsc | NIPsc | SIPsc |
| T48 | T(93), A, S (97%) | T(100) (100%) | T(94) (94%) | T(92), S (96%) | T(63), A(25), S (100%) |
| T55 | G(39), T(35), A (93%) | T(85), S (100%) | G(78), A (100%) | G(50), T, A (81%) | G(38), S(25), A (75%) |
| A78 | A(77), S, C, T (93%) | A(95), S (98%) | A(88), S (100%) | A(62), C, S (96%) | T(38) (38%) |
| G82 | G(63), A(28), S, T (94%) | G(100) (100%) | A(91), G, T (100%) | G(96) (96%) | S(38) (38%) |
| A103 | A(88), S, T, C (96%) | A(100) (100%) | A(97) (97%) | A(85), S(15) (100%) | T(50), C (62%) |
| G107 | G(61), A(32), S, T (100%) | G(97), A (100%) | G(78), A, S (100%) | A(77), T, S, G (100%) | A(100) (100%) |
| G129 | G(90), A (100%) | G(100) (100%) | G(81), A (100%) | G(81), A (100%) | G(100) (100%) |
| A130 | A(63), S(36), G (100%) | A(97), G (100%) | S(59), A(41) (100%) | S(65), A(35) (100%) | A(75), S(25) (100%) |
| G133 | A(55), G(44) (99%) | G(97) (97%) | A(97), G (100%) | A(100) (100%) | G(88), A (100%) |
| T172 | T(91), S, A (100%) | T(100) (100%) | T(97), A (100%) | T(73), S(27) (100%) | T(75), S(25) (100%) |
| S181 | A(40), S(40), G, T (93%) | S(100) (100%) | A(84), S, T (94%) | A(58), G(35), S, T (100%) | T(25), G (38%) |
| G203 | G(91), A, S (99%) | G(100) (100%) | G(100) (100%) | G(92), A (96%) | S(75), A(25) (100%) |
| S226 | S(71), T, A (97%) | S(97), T (100%) | S(75), A (94%) | T(54), S(46) (100%) | A(88) (88%) |
| G248 | G(78), A, S, C (100%) | G(100) (100%) | G(100) (100%) | A(50), G(38), S (100%) | C(38), A(25), S(25), G (100%) |
| G252 | G(97), A (100%) | G(100) (100%) | G(100) (100%) | G(100) (100%) | G(62), A(38) (100%) |
| A253 | A(66), G, T, S (97%) | A(97), G (100%) | G(72), A(25), S (100%) | A(69), T (88%) | A(62), T(38) (100%) |
| A256 | A(71), G(27) (98%) | A(97) (97%) | A(100) (100%) | G(100) (100%) | A(62), G(25), S (100%) |
a Residue numbers correspond to that of the plant aquaporin SoPIP2;1 (PDB ID: 1Z98).
b Conservation is reported for all MIP subfamilies from the structure-based sequence alignments of 105 MIP sequences from rice, maize and Arabidopsis.
c Small and weakly polar interfacial residues in plant MIP sequences and their conservation (if it exceeds 25%) are given. If the conservation is less than 25%, only the residues are reported. Group conservation of small and weakly polar residues (Gly, Ala, Ser, Thr and Cys) is shown in bold and italics.