Table 4. Suppressive mutations do not stimulate promoters of replication genes.

Promoter	Genetic context§
	PykA+	PykA−
PdnaE	4.3+/−2.2	6.9+/−1.3
PdnaC	52+/−18	41+/−21
PpolC	11+/−2	11+/−2.3

^§Promoter activity was measured in PykA+ and PykA− strains carrying (i) the lacZ ORF downstream of the promoter of dnaE, dnaC or polC and (ii) the replication genes under the control of Pspac-I. The β-galactosidase activity was measured in cells growing exponentially at 37°C in LB containing 1 mM IPTG (in these growth conditions, the IPTG-driven expression of dnaE, dnaC and polC has no effect on cell growth and morphology [5], [93], [96]. Values correspond to Miller units/mg.