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. Author manuscript; available in PMC: 2007 May 10.
Published in final edited form as: Cell. 2006 Dec 15;127(6):1209–1221. doi: 10.1016/j.cell.2006.10.039

Figure 4. NcRNAs are not required for activation of Ubx in larval imaginal discs.

Figure 4

(A). RT-PCR analysis of the amounts of ex1, ex5, bxd, and Ubx RNAs in wing, haltere and 3rd leg imaginal discs in wildtype (wt) and transgenic (N) larvae. Controls without RT are shown in the third row. rp49 was used to normalize the amount of RNA in discs between the two strains (bottom). Primer sets are the same as in Figures 5B,E.

(B) In situ hybridization of Ubx and bxd probes to wing (W), haltere (H) and 3rd leg (L) larval imaginal discs in wildtype (wt) and transgenic (N) larvae. Probes are the same as in Figure 1B,C.

(C) High magnification detection of Ubx (red) and bxd (green) nascent RNAs by fluorescent in situ hybridization in 3rd leg disc of transgenic larvae. Probes are the same as in (B).