Fig.3. Arsenic represses DNMT expression in asynchronous HaCaT cells.
(A) Relative expression of DNMT1 and DNMT3A (upper panel) and HO-1 (lower panel) in exponentially growing HaCaT cells treated with the indicated concentration of arsenic for 72 hours. (B) Relative expression of DNMT1 following 5 μM arsenite for the indicated period of time. Gene expression was measured by real-time PCR and expression level calculated relative to β-actin expression. Values are presented relative to untreated control mRNA levels for each gene. Significant differences from control are denoted by *, for p<0.05 and #, for p<0.01, by one-way ANOVA.