Table 1.
Clonal Analysis of Spleens of NFS.V† Mice with Marginal Zone Lymphomas, Prelymphomatous Marginal Zone Lesions, or No Lymphoma
| Histopathological diagnosis | Number clonal/number tested* (%) | IgH rearrangements† | |||
|---|---|---|---|---|---|
| Mono | Oligo/Mono | Oligo | Poly | ||
| Lymphoma | |||||
| MZL | 47/47 (100) | 26 (55) | 9 (19) | 12 (26) | 0 |
| MZL+ | 37/37 (100) | 25 (68) | 10 (27) | 2 (5) | 0 |
| MZL++ | 80/80 (100) | 57 (71) | 18 (23) | 5 (6) | 0 |
| Total | 164/164 (100) | 108 (66) | 37 (23) | 19 (12) | 0 |
| Prelymphoma | |||||
| EMZ | 29/35 (83) | 7 (20) | 13 (37) | 9 (26) | 6 (17) |
| Normal or Reactive‡ | 9/32 (28) | 5 (16) | 0 | 4 (12) | 23 (72) |
*Number of splenic DNAs with mono- to oligoclonal IgH rearrangements divided by number tested.
†Number of cases and (percentage) of DNA samples with clonal IgH rearrangement in each diagnostic category. DNAs were prepared from spleen, digested with EcoRI, and hybridized with JH probe. Mono, monoclonal, or biclonal, represented by a single band or two of equal intensity; Oligo, oligoclonal, typically three to five bands; Oligo/Mono, represented by several bands of variable density but with a predominant monoclonal population; Poly, polyclonal, no distinct random-sized bands but a diffuse pattern and/or concentration in 4.8- to 6.0-kb size range.
‡Reactive spleens have normal architecture and red and white pulp activity but show one or more small germinal centers within the mantle zone.