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Surface expression of podocalyxin or CD62P antigens at freshly isolated (left panel) or thrombin-treated (right panel), nonpermeabilized platelets. Both anti-podocalyxin and anti-CD62P IgGs bind to some extent to the surface of isolated platelets, indicating slight activation of the cells. A significant increase in the expression of podocalyxin or CD62P is seen after thrombin stimulation. The x axis shows fluorescence intensity.
