Figure 2.

Immunoprecipitation of nuclear extracts of L428 cells with Ki-A10 at 4°C (left) and room temperature (right) showing two neat protein bands corresponding to approximately 25 and 22 kd and a faint band near 46 kd. The signal intensity was slightly weaker when incubation was performed at room temperature. The thick bands at approximately 180 to 200 kd represent the nonreduced antibody Ki-A10 with the molecular mass of an IgG molecule. Molecular weight reference is indicated on the right.