Table 1.
Phenotype of DCs before and after Infiltration into Organotypic Cultures of SiHa Cell Line and Normal Keratinocytes Supplemented or Not with GM-CSF
| Organotypic cultures | Phenotype of DCs | Before | After 24 hours of infiltration | After 48 hours of infiltration | ||
|---|---|---|---|---|---|---|
| −GM-CSF | +GM-CSF | −GM-CSF | +GM-CSF | |||
| Normal keratinocytes | CD1a+/CD14− | 89.1 ± 0.4 | 97.1 ± 1.5 | 98.7 ± 6 | 98.3 ± 9.4 | 96.8 ± 1.5 |
| CD14+/CD1a− | 1.2 ± 0.7 | 2.7 ± 1.4 | 1.2 ± 3 | 0.9 ± 1 | 2.3 ± 0.5 | |
| CD1a+/CD14+ | 8.5 ± 1 | 0 | 0 | 1.5 ± 0.5 | 0.6 ± 0.9 | |
| SiHa | CD1a+/CD14− | 90.4 ± 0.5 | 84.5 ± 6 | 97.3 ± 1 | 85.5 ± 2 | 93 ± 4 |
| CD14+/CD1a− | 2.4 ± 0.7 | 13.7 ± 6 | 2 ± 0.8 | 9.2 ± 3 | 7.3 ± 5 | |
| CD1a+/CD14+ | 11.4 ± 0.3 | 1.3 ± 6 | 0.4 ± 0.5 | 5.2 ± 1 | 0 |
Results are expressed as percentages of labeled cells.