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. 1999 Mar;154(3):755–765. doi: 10.1016/S0002-9440(10)65322-9

Figure 6.

Figure 6.

Demonstration of type I and II neurofibromin mRNA isoforms and GAPDH mRNA by RT-PCR in TCC tissue samples and cultured TCC cell lines. Primers for NF1 mRNA amplify both type I and type II isoforms. A second primer pair was used to detect GAPDH mRNA in the same samples. Lanes 1 to 4, analyses on mRNAs isolated from four different grade 3 TCCs; lane 5, grade 2 TCC; lanes 6 to 8, RT-PCR of mRNAs derived from cell lines T24, 5637, and RT4, respectively. PstI-digested λ-DNA was used as DNA size marker (s).