Table 2.
Gene Sequences and Endonuclease Digestion Products
Gene | Position in sequence | GenBank accession No. | Product length (bp) | Restriction enzyme | Digestion fragments (bp) |
---|---|---|---|---|---|
C1q (1 intron) | 91–448 | KO 3430 | 358 | Ncol | 46, 71, 241 |
C1r | 1793–2008 | M 14058 | 216 | HaeIII | 90, 126 |
C1s (1 intron) | 860–1223 | JO 4080 | 364 | HaeIII | 114, 250 |
C2 (2 introns) | 1710–1924 | XO 4481 | 215 | BgII | 95, 120 |
C3 (2 introns) | 509–694 | KO 2765 | 186 | HaeIII | 29, 31, 126 |
C4 (2 introns) | 3228–3483 | KO 2403 | 256 | HaeIII | 78, 178 |
C5 (3 introns) | 1810–2124 | M 57719 | 315 | AluI | 53, 114, 148 |
C6 (1 intron) | 1652–1989 | JO 5064 | 338 | AluI | 32, 306 |
C7 (1 intron) | 455–702 | JO 3507 | 248 | HincII | 67, 181 |
C8 (1 intron) | 76–333 | M 17999 | 258 | AluI | 32, 226 |
C9 (1 intron) | 128–307 | KO 2766 | 180 | Sau3AI | 32, 148 |
Cyclophilin (3 introns) | 15–220 | YO 0052 | 206 | HaeIII | 53, 153 |
For primer sequences see Ref. 16 .