Table 3.
Antibodies, Sources, and Dilutions
| Antibodies | Host | Source | Dilution* |
|---|---|---|---|
| Primary | |||
| C1q | Goat | Quidel | 5k (50k) |
| C1r | Goat | ICN Biochemicals | 2k |
| C1s | Goat | Quidel | 2.5k |
| C2 | Goat | Quidel | 2k |
| C3 | Goat | Calbiochem | 10k |
| C4 | Goat | Chemicon | 10k |
| C5 | Goat | Quidel | 3k |
| C6 | Goat | Calbiochem | 2k |
| C7 | Goat | Quidel | 3k |
| C8 | Goat | Calbiochem | 5k |
| C9 | Goat | Quidel | 5k |
| C3d | Rabbit | Quidel | 5k |
| C4d | Mouse | Quidel | 3k (10k) |
| C5b-9 | Mouse | DAKO | 1k (1k) |
| sC5b-9 neo | Rabbit | Advanced Res. Tech | 2k |
| Secondary | |||
| Anti-goat HRP-conjugated IgG | Sigma | 5–10k | |
| Anti-rabbit HRP-conjugated IgG | Sigma | 5k | |
| Anti-mouse HRP-conjugated IgG | Sigma | 5–6k | |
| Anti-goat biotin-conjugated IgG | Vector | (1k) | |
| Anti-rabbit biotin-conjugated IgG | Vector | (1k) | |
| Anti-mouse biotin-conjugated IgG | Vector | (1k) |
Detection kit used: Vectastain ABC HRP Elite from Vector (2k). HRP, horseradish peroxidase.
* Dilutions without parentheses are those used in Western blots. Dilutions in parentheses are those used in immunohistochemistry.