Figure 6.

Kinase activities associated with cdk2 or p27. Cdk2-associated (upper) or p27-associated (middle) kinase activity are shown in normal lung tissue (N) and in peripheral (P) and central (C) regions of the tumor nodules of surgically resected lung tissue. Lysates (200–400 μg) were immunoprecipitated with anti-cdk2 or anti-p27 antibodies and the immunocomplex was assayed for kinase activity using glutathione S-transferase-RB fusion protein as a substrate. The result of immunoblotting analysis with anti-AE1 antibody (lower) showing standardization of the amount of protein from epithelial cells in three areas described above. IP, immunoprecipitation; IB, immunoblotting.