Figure 2.

ECM does not affect expression of receptor tyrosine kinases. GECs on collagen (C) or plastic (P) were solubilized in detergent-containing buffer. For EGF-R, samples (125 μg of protein) were subjected to SDS-PAGE and immunoblotting with anti-EGF-R receptor antibody. The arrow points to the 170-kd EGF-R. For Met and FGF-R2, samples (1.5 mg of protein) were immunoprecipitated with receptor-specific antibodies before SDS-PAGE and immunoblotting with receptor-specific antibodies. In the Met blot, the arrow points to the 145-kd Met. The upper band (∼190 kd) probably represents the unprocessed Met precursor, and the lower band may be a degradation product. In the FGF-R2 blot, the arrow points to the 150-kd (3 Ig domain) FGF-R2. The lower band at ∼125 kd probably represents the 2 Ig domain FGF-R2. 17