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Overexpression of V12Ras. GECs adherent to collagen were transfected with a constitutively active Ha-Ras gene (V12Ras). Clones that proliferated on plastic were selected and assessed for expression of Ras protein by SDS-PAGE and immunoblotting (25 μg of protein per lane). Expression of endogenous Ras in parental (untransfected) GECs is not readily detectable by immunoblotting. Four clones express high (R514), intermediate (R25, R34), or low levels of V12Ras (R311).
