Table 1.
Anchorage Dependence of Proliferation in V12Ras-Transfected GECs
| Number of colonies | Number of wells | |
|---|---|---|
| Parental GECs | 0 ± 0 | 6 |
| Ras-transfected GECs | ||
| R514 | 4.0 ± 0.7* | 6 |
| R25 | 0 ± 0 | 3 |
| R34 | 0 ± 0 | 3 |
| R311 | 0 ± 0 | 3 |
GECs were plated in soft agar in K1 medium (10,000 cells/60-mm well). The number of colonies was determined in 10 randomly selected, low-power microscopic fields after 14 days of culture. Significant differences were present among groups (P < 0.0001, ANOVA).
*Only clone R514, which stably expresses a high level of V12Ras, proliferated in an anchorage-independent manner (P < 0.0001 versus other groups).