Table 2.

Percentage of Cells with Chromosomal Alterations Isolated from 32-Week-Old Mice

	Chromosome number
	1-	4-	7-	9-	12q	14-	X-
Control	<1	6.0 ± 3.0	<1.0	6.0 ± 3.0	<1.0	<1.0	<1.0
Tumors	80.0 ± 26	98.0 ± 3	86.0 ± 10	75.0 ± 13	86.0 ± 7	72.0 ± 8	98.0 ± 20

Eleven hepatocellular carcinomas isolated from the c-myc/TGF-α mice and hepatocytes from five age-matched (Cd 1 × B6CBA)F1 mice were cultured as described in Materials and Methods, and metaphase preparations were made from the first two divisions. The metaphase spreads from 11 tumors and hepatocytes from five age-matched controls were hybridized with chromosomal paints for chromosomes 1, 4, 5, 7, 8, 9, 11, and 12 and the X chromosome. At least 20 metaphase spreads were analyzed for each chromosome paint. The inverted DAPI from each hybridized metaphase spread was analyzed to determine the band region that was altered. A minimum of 20 G-banded metaphase spreads were karyotyped for each tumor and control. The data are expressed as the mean percent ± SD of cells with a specific aberration.