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. 1999 Apr;154(4):1159–1169. doi: 10.1016/S0002-9440(10)65368-0

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Copyright © 1999, American Society for Investigative Pathology

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Figure 6. — Immunohistochemical detection of canine IL-1Ra in synovium of rabbit OA knees. A: A 4-week OA rabbit. B: An 8-week OA rabbit injected with the control plasmid showing no specific staining for canine IL-1Ra. C: An 8-week OA rabbit treated with 500 μg of IL-1Ra plasmid. D: An 8-week OA rabbit treated with 1000 μg of IL-1Ra plasmid showing some staining in chondrocytes mainly in the superficial layer. Controls using specimens from 8-week old rabbits treated with 500 μg (E) and 1000 μg (F) of IL-1Ra plasmid performed with an immune absorbed serum using a 20-fold mass equivalent to the purified antigen showed no specific staining, Original magnification, ×250.