Figure 8.

PCR analysis of rabbit synovial membranes for the presence of the dog IL-1Ra gene. Plasmids were injected into the knee joints of rabbits as described in Materials and Methods. DNA was extracted from the synovial membranes of untreated (Group 1; n = 3) and treated (Group 4; n = 4) rabbits and subjected to PCR analysis to detect the cloned cDNA dog IL-1Ra. MW, molecular weight markers (pGEM markers, Promega, Madison, WI); −, negative control (reaction mixture without template DNA); +, positive control (plasmid IL-1Ra used as DNA substrate). The size of the specific amplified DNA fragment is 427 bp.