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. 1999 Apr;154(4):1097–1104. doi: 10.1016/S0002-9440(10)65362-X

Figure 2.

Figure 2.

Immunofluorescent analysis of wound-derived cells harvested 24 hours after injury demonstrating strong iNOS antigen expression among infiltrating macrophages and undetectable or weak staining of PMNs. A: anti-iNOS polyclonal Ab; B: Anti-rat macrophage Ab; C: Dual staining demonstrating iNOS expression in macrophages; D: Phase contrast of cells shown in A to C; E: Anti-iNOS polyclonal Ab; F: Anti-rat PMN Ab; G: Dual staining demonstrating minimal iNOS expression in PMNs; H: Phase contrast of cells shown in E to G. Bar, 10 μm.