Table 3.

l-Arginine Metabolism by Freshly Harvested and Overnight Cultured Day 10 Wound-Derived Macrophages

Macrophage	Citrulline production (nmol/h/106 cells)
Freshly harvested	0.18 ± 0.07
Overnight cultured	4.01 ± 0.33

Wound-derived macrophages obtained 10 days after injury were cultured in the presence of 1 mmol/L l-arginine and 0.1 μCi/ml [guanido ¹⁴C]l-arginine for 2 hours either immediately after harvest or after overnight culture. The appearance of radiolabel in l-citrulline, an indicator of iNOS activity, in culture supernatants was determined as indicated in Materials and Methods.