Figure 2.

Time dependent induction of apoptosis in COS-1 cells expressing hIAPP. COS-1 cells were transfected with 15 μg of either the pMT2-hIAPP (A) or pMT2-hIAPP^anti (B) expression vectors and cultured as described (Materials and Methods). At 24, 48, 72, and 96 hours after transfection, cells were assessed for apoptosis and necrosis by annexin-V and 7-AAD labeling with FACS analysis. Histogram profiles for annexin-V labeling for each group are shown with the apoptotic cells (annexin-V positive) delineated by the bar (M1).