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. 1999 Apr;154(4):1077–1088. doi: 10.1016/S0002-9440(10)65360-6

Figure 3.

Figure 3.

Expression of the hIAPP polypeptide and its amyloidogenic domain are required for induction of apoptosis in COS-1 cells. A: Expression of the hIAPP antisense mRNA in COS-1 cells blocks hIAPP-induced apoptosis. COS-1 cells were transfected with the pMT2, pMT2-hIAPPanti, pMT2-hIAPP, or equimolar concentrations of both pMT2-hIAPP and pMT2-hIAPPanti expression vectors and cultured as described (Materials and Methods). After 96 hours the cells were assessed for apoptosis and necrosis by labeling with annexin-V (cross-hatched bars) and 7-AAD (shaded bars) and analyzed by FACS. B: Expression of hIAPP containing its amyloidogenic domain but not a mutant hIAPP or rIAPP, lacking the amyloidogenic domain, leads to apoptosis of transfected COS-1 cells. Cells were transfected with 15 μg of pMT2, pMT2-hIAPPanti, pMT2-hIAPP, pMT2-hIAPPmut, or pMT2-rIAPP expression vectors. Apoptosis was assessed by annexin-V binding and FACS analysis at 72 (open bars) or 96 hours (shaded bars). The data are from three independent experiments; data marked with asterisks were statistically different from control (P < 0.05) by multivariate analysis of variance and post hoc Bonferroni t-test.