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. 2002 Sep;12(9):1434–1444. doi: 10.1101/gr.130502

Figure 6.

Figure 6

Effect of copy-up mutations in the trfA gene on DNA amplification. All host strains carry the same pBAC/oriV plasmid with a 108-kb DNA insert (pCG275). Growth conditions and the induction of TrfA synthesis by L-arabinose (A) are described in Methods. DNA samples prepared by phenol extraction and ethanol precipitation were digested with SmaI and run on a 0.6% agarose gel. (Lanes 1,3,5,7) LB medium (LB); (lanes 2,4,6,8) LB + 0.01% A. (Lanes 1,2) Strain JW439 containing the trfA254 mutation; (lanes 3,4) strain JW499 containing the trfA173 mutation; (lanes 5,6) strain JW500 containing the trfA171 mutation; (lanes 7,8) strain JW501 containing the trfA250 mutation. Amplifications (lanes 2,6,8) of plasmid + 108-kb insert are estimated to be up to 30-fold.