Table 2.

List of Strains

E. coli strains	Pertinent genotype
DH10Ba	F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL
JW366	DH10B, with pJW349 (trfA203) integrated at the attB site; see Figs. 2 and 3
JW371	JW366 [pBAC/oriV]
JW378	JW366 [pBAC/oriV+20-kb insert (pJW378)]
JW389	JW366 [pBAC/oriV+40-kb insert (p67)]
JW390	JW366 [pBAC/oriV+80-kb insert (p69)]
JW427	DH10B with pJW424 (trfA254) integrated at the attB site; see Table 1 and Fig. 2
JW439	JW427 [pBAC/oriV+108-kb insert (pCG275)]
JW450	JW427 [pBAC/oriV+122-kb insert (pCG274)]
JW461	DH10B with pJW458 (trfA173) integrated at the attB site; see Table 1 and Fig. 2
JW462	DH10B with pJW459 (trfA171) integrated at the attB site; see Table 1 and Fig. 2
JW463	DH10B with pJW457 (trfA250) integrated at the attB site; see Table 1 and Fig. 2
JW467	JW461 [pJW378]
JW468	JW462 [pJW378]
JW471	JW461 [pBAC/oriV+140-kb insert (pCG273)]
JW472	JW462 [pBAC/oriV+140-kb insert (pCG273)]
JW480b	GenHogs (Invitrogen) with pJW424 (trfA254) integrated at the attB site, see Table 1 and Fig. 2
JW499	JW461 [pBAC/oriV+108-kb insert (pCG275)]
JW500	JW462 [pBAC/oriV+108-kb insert (pCG275)]
JW501	JW463 [pBAC/oriV+108-kb insert (pCG275)]
JW526c	Stbl2 cells (Life Technologies/Invitrogen) with pJW424 (trfA254) integrated at the attB site; see Table 1 and Fig. 2
JW550	JW463[pIndigoBAC/oriV]

^aThe E. coli DH10B strain is described in Invitrogen Catalog 2002, p. 229. 

^bThe GeneHogs strain is described in Invitrogen Catalog 2002, p. 230. 

^cThe Stbl2 strain is the gal⁺ lon⁻ parental strain of Stbl4 described in Invitrogen Catalog 2002, p. 234. 

Brackets indicate plasmid, as carried in the host strain. Plasmid designations (see Table 1) or mutant numbers are in parentheses. Δ( ), deletion (followed by deleted genes, in parentheses, and number).