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. 1999 Apr;154(4):1149–1158. doi: 10.1016/s0002-9440(10)65367-9

Figure 2.

Figure 2.

IL-1β and TNF-α induce transient VCAM-1 expression on fibroblast-like synoviocytes. Cells were treated with either medium only (open circles) , or medium plus IL-1β (10 ng/ml) (closed circles), or medium plus TNF-α (10 ng/ml) (open squares) for 1, 2, 3, or 7 days. VCAM-1 cell-surface expression was determined by flow cytometry using mAb BBA-5 as primary antibody. Samples were analyzed using a FACScan and the results expressed as corrected MFI. Data are presented as the mean ± SE of six experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (compared with medium-only at the respective time point).