Figure 3.

Production of reactive oxygen species and lipid peroxidation metabolites in normal versus Mpv17−/−glomeruli. A: Production of reactive oxygen species by equal numbers of isolated glomeruli of control BALB/c and Mpv17−/− mice, as determined by the luminol method. Glomeruli of Mpv17−/− mice produce approximately the double amount of oxygen radicals of controls. Production of radicals is expressed as counts (determined in the scintillation counter) per milligram of glomerular protein. B: The amount of lipid peroxidation adducts was determined by the dithiobarbituric assay specific for MDA adducts. Mpv17−/− kidney cortex contains more than twice the amount of MDA adducts when compared to normal controls. The heights of the columns represent the mean values of 5 experiments and the bars indicate standard deviations. In both assays the differences were statistically significant (unpaired t-test).