Figure 5.

Cellular localization of C10 RNA expression in the spinal cord of control mice (A, B) or mice with MBP-EAE (C, D) or in the brain of GFAP-IL3 mice (E, F). Double-labeling experiments used antisense riboprobe for C10 RNA and immunostaining for GFAP (to label astrocytes) or binding of RCA-1 lectin (to label macrophage/microglia). In the GFAP-IL3 and EAE specimens numerous cells positive for C10 (arrows) colabeled with the RCA-1 lectin (C and E). In contrast, no GFAP-positive cells (D and F) were observed that were also positive for C10. Hybridization of the C10 probe to non-GFAP-labeled cells was conspicuous (arrows).