Representative findings after genetic analysis of various histological components from different mature ovarian teratomas. After PCR, the amplification products were separated using 8% polyacrylamide gels; each lane depicted below represents a separately procured tissue sample. A: Case 1, various histological components being consistently homozygous with markers Int-2, D1S1646, and D3S2452; normal host ovarian stroma is heterozygous. lane 1, normal ovarian stroma; lane 2, squamous epithelium; lane 3, neuroglial tissue; lane 4, sebaceous gland; lane 5, striated muscle; lane 6, neuroglial tissue; lane 7, sebaceous gland; lane 8, neuroglial tissue; lane 9 , sebaceous gland; lane 10, squamous epithelium; lane 11, squamous epithelium; lane 12, neuroglial tissue; lane 13, squamous epithelium) B: Case 7, evidence of genetic recombination. Lane 1, Normal ovarian tissue; lane 2, normal ovarian tissue; lane 3, squamous epithelium; lane 4, lymphoid aggregate in squamous epithelium. Teratomatous squamous epithelium is homozygous with Ank-1 but heterozygous with D9S303; in contrast, normal ovarian tissue and lymphoid aggregate are heterozygous with both markers.