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. 1999 Apr;154(4):987–991. doi: 10.1016/S0002-9440(10)65350-3

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Copyright © 1999, American Society for Investigative Pathology

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Figure 3. — A: Case 4, analysis of lymphoid aggregate (lane 7, marked by asterisk) reveals two (heterozygous) alleles with markers D1S1646, D9S303, and Ank-1, whereas microdissected teratomatous tissues of variable heterotopic differentiation are consistently homozygous.lane 1, normal ovarian stroma; lane 2, normal ovarian stroma; lane 3, cerebellum; lane 4, cerebellum; lane 5, squamous epithelium; lane 6, neuroglial tissue; lane 7, lymphoid aggregate in squamous epithelium; lane 8, sebaceous glands; lane 9, pilar follicles. B: Case 2, various histological components being consistently homozygous with markers D9S303, Ank-1, Int-2, and IFNA. Normal host ovarian stroma is heterozygous; however, a second allele of variable intensity can be observed in samples 6, 7, and 8 (marked by asterisks) that represent different teratomatous tissues with lymphoid cells. Samples 14 and 15 (marked by asterisks) represent selectively procured lymphoid aggregates with two alleles of equal intensity. Lane 1, normal ovarian stroma; lane 2, normal ovarian stroma; lane 3, sebaceous gland; lane 4; sebaceous gland; lane 5, squamous epithelium; lane 6, neuroglial tissue; lane 7, squamous epithelium with lymphoid cells; lane 8, sebaceous glands with lymphoid cells; lane 9, neuroglial tissue; lane 10, cartilage; lane 11, sebaceous gland; lane 12, respiratory epithelium; lane 13, sebaceous glands; lane 14, lymphocytes associated with respiratory epithelium; lane 15, lymphocytes associated with respiratory epithelium; lane 16, sebaceous glands; lane 17, respiratory epithelium; lane 18, respiratory epithelium. C: Case 3. Thymic tissue obtained from case 3 reveals homozygosity with markers Ank-1 and Int-2. Normal host ovarian stroma is heterozygous T, thymus; N, normal ovarian tissue.