Table 1.
The Addition or Neutralization of Angiogenic CXC Chemokines Does Not Change the Growth of PC-3 or Du145 Cells in Vitro
| PC-3 | Du 145 | |
|---|---|---|
| Vehicle | 13,596 ± 321 | 14,910 ± 464 |
| +IL-8 (100 ng/ml) | 13,520 ± 443 | 16,836 ± 256 |
| +GRO-α (100 ng/ml) | 14,585 ± 533 | 15,192 ± 1485 |
| +anti-IL-8 Ab (1:10) | 11,438 ± 368 | 11,786 ± 270 |
| +anti-GRO-α (1:10) | 14,912 ± 850 | 13,998 ± 1232 |
| +NRS (1:10) | 15,047 ± 2032 | 15,278 ± 660 |
PC-3 or Du145 cells were cultured for 24 hours in the presence or absence of the indicated concentrations of recombinant angiogenic CXC chemokines or a 1:10 dilution of the neutralizing rabbit anti-murine CXC chemokine antisera before being pulsed with 3-H thymidine for 16 hours. Data were similar when 1 and 10 ng/ml chemokine were added or when the neutralizing antisera was used at a 1:100 dilution. Furthermore, data were similar when cultured for 48 hours prior to pulsing. Data shown represent the mean CPM ± SEM of at least six wells per experimental group.