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. 1999 May;154(5):1335–1343. doi: 10.1016/S0002-9440(10)65387-4

Figure 2.

Figure 2.

A: Identification of differential gene expression in LNCaP (L) (Lanes 2 and 3) and LNCaP-r (R) (Lanes 4 and 5) cells by SSH before cloning. PCR products of either subtracted (S) (Lanes 2 and 4) or unsubtracted (U) (Lanes 3 and 5) cDNA libraries are separated on a 1.5% TAE agarose gel. Lane 1: φX174 HaeIII markers; Lane 6: positive control for subtraction (φX174 HaeIII markers in placental cDNA). Primers and PCR conditions are described in Materials and Methods. B: An example of colony PCR, the products of which range from 0.2–0.8 kb. C: Reverse Northern screening assay for differentially expressed genes. Replica DNA dot blots were prepared and probed with either DNA from LNCaP (left panel) or LNCaP-r (right panel) subtracted libraries. Clones 49L and 17R are circled on left and right blots, respectively.