Table 1.
Exon | Primer name | Primer sequence | PCR product size | TM* |
---|---|---|---|---|
1 | 1S | gccaccagcagcttctgcc | 187 | 55 |
1AS | ctaagagagtgacagaaagg | |||
2 | 2S1 | accttttattactccagcta | 191 | 48 |
2AS1 | ttcctgtatacgccttcaag | |||
2S2 | caaacattattgctatggga | 169 | 48 | |
2AS2 | atctttttctgtggcttaga | |||
3 | 3S | gctcattttgttaatgg | 135 | 55 |
3AS | tagaagatatttgcaagc | |||
4 | 4S | ataaagattcaggcaatgtt | 184 | 48 |
4AS | atcgggtttaagttatacaa | |||
5 | 5S1 | agtttgtatgcaggcaatgtt | 240 | 55 |
5AS1 | atcattacaccagttcgtccc | |||
5S2 | gcagcaattcactgtaaagc | 179 | 55 | |
5AS2 | cagatccaggaagaggaaagg | |||
6 | 6S1 | ttttcaatttggcttctctt | 143 | 48 |
6AS1 | catcttgtgaaacaacagtgcc | |||
6S2 | cctgttaaagaatcatctgg | 137 | 48 | |
6AS | ctgttccaatacatggaagg | |||
7 | 7S1 | ttcctgtgaaataatactgg | 179 | 55 |
7AS1 | gaactctactttgatatcacc | |||
7S2 | ttcatgtactttgagttccc | 131 | 55 | |
7AS2 | ccttattttggatatttctccc | |||
8 | 8A1 | tgcaaatgtttaacataggtga | 170 | 55 |
8AS1 | ttccttgtcattatctgcacg | |||
8S2 | caagaaatcgatagcatttgc | 181 | 55 | |
8AS2 | atacatacaagtcaacaaccccc | |||
9 | 9S1 | taagatgagtcatatttgtggg | 179 | 55 |
9AS1 | cagagtcagtggtgtcaga | |||
9S2 | tagaggagccgtcaaatcca | 194 | 48 | |
9AS2 | catggtgnnttatccctctt |
*TM, annealing temperature (°C).