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Comparision of signal intensity trends for Sybr Green I, Fluorescence resonance energy transfer (FRET), and induced (i)FRET. Melting curves are shown for the three indicated assay formats. Fluorescence measurements were recorded at the empirically determined emission maxima for respective dyes: for SYBR Green I detection: 541–545 nm, for FRET detection (FAM): 541–545 nm, and for iFRET detection (ROX): 611–615 nm.
