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. 1999 May;154(5):1439–1447. doi: 10.1016/s0002-9440(10)65398-9

Figure 3.

Figure 3.

Flow cytometric analysis of 7-AAD staining in anti-CD95 (Fas)-treated CHD cell lines. Increased cell death was observed after 72 hours of treatment with 500 ng/ml agonistic CD95 mAb (CH11) in HS445 and L428 cell lines compared to untreated control cells. Pretreatment of cells with caspase-3 peptide inhibitor, DEVD-FMK, significantly decreased cell death in anti-CD95-treated cells to near background levels. In contrast, no significant increase in cell death was observed after anti-CD95 treatment of KMH2 cells as compared to untreated cells. The x axis represents fluorescence intensity (log scale) and the y axis represents relative cell number. These data are representative of at least three separate experiments performed.