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. 1999 May;154(5):1439–1447. doi: 10.1016/s0002-9440(10)65398-9

Figure 4.

Figure 4.

TUNEL assay for apoptosis. Compared to untreated cells (A), the L428 cell line displayed a considerable increase in apoptosis (dark brown/black cells) after 72 hour incubation with 500 ng/ml agonistic CD95 (Fas) mAb (B). Pretreatment of L428 cells with 10 μM DEVD-FMK decreased the number of apoptotic cells to near baseline levels (C). Stimulation of KMH2 cells with anti-CD95 mAb with or without DEVD-FMK pretreatment showed no increase in apoptosis compared to untreated cells (data not shown).