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Apoptosis Rates Induced in Anti-CD95 mAb Treated or Untreated CHD Cell Lines as Determined by TUNEL Assay
| CHD cell line | Apoptosis* | ||
|---|---|---|---|
| Untreated | Anti-CD95 | Anti-CD95+ DEVD-FMK | |
| HS445 | 7.5% | 20.5% | 9.9% |
| L428 | 7.8% | 30.2% | 10.8% |
| KMH2 | 1.0% | 1.3% | 1.0% |
*1 × 106 cells were untreated or treated with 500 ng/ml anti-CD95 mAb (CH11) for 72 hours with or without pretreatment with 10 μM caspase-3 peptide inhibitor, DEVD-FMK. Apoptosis was quantitated by TUNEL staining as described in Materials and Methods.
