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. 1999 May;154(5):1367–1379. doi: 10.1016/S0002-9440(10)65391-6

Figure 5.

Figure 5.

Light (A and B) and electron microscopic (C and D) analyses of the developing yolk sac vasculature at day 9.5 p.c. harvested from conceptuses of control (A and C) and streptozotocin-treated diabetic mothers (B and D). A: High-power representative light micrograph of the terminal yolk sac vasculature capillaries observed in a conceptus harvested at day 9.5 p.c. from a normoglycemic mother. Note the vessel lined by flattened endothelial cells (arrowheads) containing blood cells in the lumen, bordered by the endodermal cells on one aspect (double arrows) and flattened, elongated mesenchymal cells (pericytes) on the other aspect (arrows) in close contact with the abluminal surfaces of the endothelium. Scale bar, 20 μm. B: High-power representative light micrograph of the terminal yolk sac vasculature capillaries observed in a conceptus harvested at day 9.5 p.c. from a streptozotocin-treated diabetic mother. Note the vessel lined by endothelial cells (arrowheads), bordered by endodermal cells on one aspect (double arrows) and plump, rounded mesenchymal cells (pericytes) on the other aspect (arrows) in loose contact with the abluminal surfaces of the endothelium. Several blood cells are present in the lumen. Scale bar, 20 μm. C: Representative transmission electron micrograph of the terminal yolk sac vasculature capillaries observed in a conceptus harvested at day 9.5 p.c. from a normoglycemic mother. Note the vessel lined by flattened endothelial cells (arrowheads) bordering the lumen and the flattened, elongated mesenchymal cells (pericytes) in close contact with the abluminal surfaces of the endothelium (arrows). Scale bar, 7.3 μm. D: Representative transmission electron micrograph of the terminal yolk sac vasculature capillaries observed in a conceptus harvested at day 9.5 p.c. from a streptozotocin-treated diabetic mother. Note the vessel lined by endothelial cells (arrowheads) bordering the lumen and the plump, rounded, mesenchymal cells (pericytes) in loose contact with the abluminal surfaces of the endothelium. Scale bar, 7.3 μm. Experiments were performed at least five times with separate litters.