Table 1.

Summary of the Particle Exclusion Assay Data Using LP9, NIH:OVCAR5, and SKOV3 Cells

Cell line	No treatment	Hyaluronidase treatment	RCS-PG treatment	Hyaluronidase treatment followed by RCS-PG treatment
LP9	+++	−	++++	−
NIH:OVCAR5	++	+	+++	−
SKOV3	−	−	+	−

LP9, NIH:OVCAR5, and SKOV3 cells were seeded into six-well plates at a low density and then cultured for 24 hours. The pericellular matrix was visualized by the particle exclusion assay using fixed goat RBCs (see Figure 7 ▶ ). Cells were incubated in the assay buffer for 1 hour before adding the RBCs or treated with either hyaluronidase, RCS-PG, or hyaluronidase for 1 hour followed by incubation with 2 mg/ml RCS-PG. The size of the pericellular matrices visualized under these conditions were scored as follows: −, absent; +, small; ++, medium; +++, large; ++++, very large.