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Analysis of CSMC for expression of smooth muscle-specific myosin heavy chain and 17-kd light chains mRNAs. Smooth muscle-specific PCR primers (SM1/SM2; Lane 1) and (LC17a/LC17b; Lane 2) were used to competitively amplify cDNA derived from CSMC. Human smooth muscle α-actin was also amplified (Lane 3) as described in Materials and Methods. A 100-bp DNA ladder was used as a molecular size standard (M).
