Table 1.
Nucleotide Sequences of the Primers Used for RT-PCR
| Transcript | Sequence | Accession No. | Expected PCR product (bp) |
|---|---|---|---|
| Cathepsin S | 5′-CGGGATCCATGAAACGGCTGGTTTGTGTG-3′ | M90696 | 996 (full length) |
| 3′-GGGGTACCCTAGATTTCTGGGTAAGAGGG-5′ | |||
| MMP-1 | 5′-ATGCACAGCTTTCCTCCACTGCTGCTGCTG-3′ | X54925 | 1410 (full length) |
| 3′-TCAATTTTTCCTGCAGTTGAACCAGCTATT-5′ | |||
| MMP-3 | 5′-ATGAAGAGTCTTCCAATCCTACTGTTGCTG-3′ | J03209 | 1434 (full length) |
| 3′-TCAACAATTAAGCCAGCTGTTACTCTTCAA-5′ | |||
| MMP-9 | 5′-CTCTATGGTCCTCGCCCTGAACCTGAGCCA-3′ | J05070 | 801 (nt 1343-2143) |
| 3′-CTAGTCCTCAGGGCACTGCAGGATGTCATA-5′ | |||
| TIMP-1 | 5′-ATGGCCCCCTTTGAGCCCCTGGCTTCTGGC-3′ | X03124 | 624 (full length) |
| 3′-TCAGGCTATCTGGGACCGCAGGGACTGCCA-5′ | |||
| TIMP-2 | 5′-ATGGGCGCCGCGGCCCGCACCCTGCGGCTG-3′ | J05593 | 663 (full length) |
| 3′-TTATGGGTCCTCGATGTCGAGAAACTCCTG-5′ |
Primer sequences used to generate cDNA probes used in these studies and the GenBank accession numbers for the sequences from which they were derived. All probes were sequenced to verify that they encoded the cognate cDNAs.