Figure 2.

Mutations and deletions of Fas gene in cutaneous MM. SSCP (A and D) and sequencing analysis (B and C) of DNA from tumors (Lane T) and normal tissues (Lane N). A: Part of exon 9 was amplified using primer set 9A. Left, SSCP of DNA from tumor (T) of case 5 shows wild-type bands and additional aberrant bands as compared to SSCP from normal lymphocytes (N). Right, SSCP of DNA from tumor (T) of case 9 shows only 2 aberrant bands without any wild-type bands as compared to SSCP from normal lymphocytes (N). B: Sequencing analysis from aberrant band of case 5. There is a G-to-A transition at nucleotide 963 (arrow) in tumor tissue as compared to normal tissue. C: Sequencing analysis from aberrant band of case 9. There is an A-to-G transition at nucleotide 991 (arrow) in tumor tissue as compared to normal tissue. D: Detection of allelic loss by amplifying a region encompassing the biallelic polymorphism, −670, in the Fas promoter. Left, representative SSCP showing retention of heterozygosity. Right, a representative pattern of LOH. Loss of two bands was observed in DNA from tumor cells (T) compared to the SSCP from normal cells (N).